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Fig. 3. Eda-A1 recombinant protein increased the size and caused fusions of the
hair follicles in in vitro cultures of embryonic skin. Hair follicles of the
cultured back skin samples were analysed by whole-mount in situ hybridisation
with ß-catenin probe. E13 skin explants showed bigger and fused hair
follicles after 1 day in culture with the conditioned media from cells
producing recombinant Eda-A1 protein (A), compared with normal hair follicles
seen in cultures with Y343 control media (B). Similar effect was seen also
after two days of culture (C,D). Older explants of E14 skin showed no effects
after 1 day in culture with Eda-A1 medium (E): the hair follicles were of a
similar size to those of the control cultures (F). After 3 days of culture
with Rec Eda-A1 media (G), the hair follicles were clearly bigger than in
control cultures (H).
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