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Fig. 5. Bmp4 inhibits placode formation, despite the presence of Eda-A1. E13 wild-type skin explants were grown for 24 hours in the presence of 2 µg/ml of Eda-A1 and analysed for placode formation by whole-mount in situ hybridisation with ß-catenin (data not shown) or Shh probe. BSA control beads (A; 0/12 explants) had no effect, whereas two agarose beads soaked in 20 ng/µl (B; 11/12 explants), 75 ng/µl (C; 10/10 explants) or 500 ng/µl (D; 13/13 explants) of Bmp4 placed on top of each explant inhibited placode formation and enlargement in the vicinity of the bead.





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