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Fig. 6. Eda-A1 overexpression caused enlargement of the first molar placode at E12
and an extra molar placode in E13 lower jaws. (A-F) Top views of lower jaws.
The odontogenic molar (red arrows in A and B) and incisor (blue arrows in A
and B) fields were visualised in E11 embryonic lower jaws by Pitx2
expression. No difference was seen between wild-type (A) and
K14-Eda-A1 transgenic (B) embryos. At E12, the dental placodes were
visualised by Shh expression (C,D). The molar placodes were larger in
transgenic embryos when compared with controls (arrows in C,D). At E13, extra
placodes (blue arrow) were located in front of the transgenic molar placodes
(red arrow in F). However, a small Shh-positive placode was also
detected in wild-type embryos (blue arrow in E). The size measurements of E12
mandibular molar placodes of K14-Eda-A1 and control mice are
presented as a box plot presentation (G). The size measure is a product of
width and length of a placode. The height of the box presents 50% of the
results. Mean is shown as a horizontal line inside the box and 95% confidence
limits are shown by the lines outside the box. The difference between groups
is statistically significant (P=0.03) according to the Mann-Whitney
U-test. Measurements were made on three litters of mice. Number of placodes
measured: K14-Eda-A1 n=23; wild-type littermates, n=18.
Scale bars: 1 mm.
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