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Fig. 2. Wing size correlates negatively with brk expression. All the discs
are doubly stained for GFP and brk (anti-ßgal). The right panels
show brk expression in red. For simplification, the labels present
only a partial notation of the genotype (see Materials and methods). (A,B) A
nub-Gal4>UAS-tkvQD UAS-GFP disc with a large wing pouch
that has little or no brk activity. (C,D)
nub-Gal4>UAS-GFP of normal size and normal brk activity.
(E,F) The reduction of Dpp pathway activity in
nub-Gal4>UAS-tkvDN UAS-GFP results in a small wing
pouch associated with high brk levels. (G,H) Further diminution of
Dpp pathway activity in nub-Gal4>UAS-dad UAS-GFP produces a
smaller wing pouch associated with an expansion of brk expression
that covers the whole of the wing pouch.
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