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Fig. 2. CONT is expressed in ectodermal epithelia and glial cells of the PNS in
Drosophila embryos. (A-D) A confocal section of a whole-mount stage
16 wild-type embryo triple stained with anti-CONT (A, blue), anti-NRX IV (B,
red) and anti-NRG (C, green). CONT is expressed in all the ectodermally
derived epithelial cells, including epidermis, salivary glands, trachea,
hindgut and peripheral glial cells. The focus is on the midline of the CNS and
the exiting axon tracts and parts of the epidermis. The merged image of the
three panels is shown in D. Scale bar: 50 µm for A-D. (E-H) Part of the CNS
midline and peripheral glial cells at a higher magnification shows that CONT
(E, blue) and NRG (G, green) are not expressed in midline glial cells where
NRX IV is strongly expressed (F, red, see arrow pointing to the midline glial
cells). The roots of the glial cells, which ensheath axonal commissures in the
CNS, express all three proteins (E-H, arrowheads). In the peripheral nerves,
the expression pattern of all three proteins overlap (asterisks). (I-L) A
region of the epidermal epithelial cells at a higher magnification
demonstrates that CONT (I, blue), NRX IV (J, red) and NRG (green) are
co-expressed at SJs (L, merged color white). NRG expression extends more
basolaterally (arrow in K and L), whereas CONT and NRX IV are restricted to SJ
domains. The arrowheads in I-L indicate overlapping expression along the
peripheral nerve. (M-P) Part of the hindgut at a higher magnification also
shows that CONT (M, blue), NRX IV (N, red) and NRG (O, green) are concentrated
at SJs, but that NRG extends more basolaterally (arrowheads in O and P). Scale
bar: 16 µm for E-P.
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