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Fig. 6. The paracellular barrier is compromised in cont mutant embryos. (A) cont/GFP heterozygous late stage embryo injected with rhodamine-labeled dextran into the body cavity reveals an intact paracellular barrier as the dye fails to penetrate into the lumen of the salivary glands 20 minutes after injection (arrow). (B) cont mutant embryos injected with the dye into the body cavity reveals breakdown of the paracellular barrier as the dye penetrates into the lumen of the salivary glands within 18 minutes (arrow). (C) cont mutant embryos injected with the dye into the body cavity reveals breakdown of the paracellular barrier in the tracheal lumen (arrowhead). The tracheal tube is convoluted in the cont mutant embryo. The salivary gland filled with the dye is indicated by an asterisk. Scale bar: 20 µm for A,B; 60 µm for C.

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