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Fig. 8. Cell-surface expression of CONT depends on NRX IV. Neuroblastoma N2a cells
were transfected with NRX IV or CONT, or co-transfected with both constructs.
(A) NRX IV is efficiently transported to the cell surface in the absence of
CONT. (B-D) When transfected alone, CONT is not expressed at the plasma
membrane. Double-immunostaining of CONT (B, red) and the ER marker BiP (C,
green). The merged image (D) shows that CONT is retained in the ER
(arrowheads). (E-H) Co-transfection of CONT (E) and NRX IV (F) in N2a cells
results in expression of CONT protein at the plasma membrane. (G,H)
Cell-surface expression of CONT in N2a cells co-transfected with CONT and NRX
IV. Cells were fixed, permeabilized with Triton X-100 and processed for
immunofluorescence staining in A-F. Immunostaining for CONT was carried out on
living cells in G and then cells were fixed, permeabilized and processed for
NRX IV immunostaining (H). Scale bar: 10 µm.
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