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Fig. 2. ash2 downregulates the Egfr pathway during vein development. (A) Lack of wing veins in rho^ve vn¹ homozygous flies. (B) In the triple mutant rho^ve vn¹ ash2¹¹²⁴¹¹, the loss of veins is rescued. (C) top¹/top^3C81 adult wing that lacks the a-cv and the middle part of L4. (D) The combination top¹/top^3C81 with one copy of the ash2^I1 allele restores the wild-type vein pattern. (E) The gain-of-function Elp^B1 results in ectopic vein tissue in distal L2. (F) Elp^B1; ash2¹¹²⁴¹¹ individuals show a reduction in wing size, with notches appearing in the posterior margin and extra vein tissue (extra crossveins and a proximal L2-L3 fusion). (G) rl^Sem/+ flies develop wings with ectopic branches of veins. (H) Ectopic vein tissue is enhanced in rl^Sem; ash2^I1 transheterozygous flies. All wings are shown at the same magnification.

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