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Fig. 5. ash2 is required for activation of bs. (A) Wings of
heterozygous bs03267 flies display a small ectopic vein in
intervein region D. (B,C) bs03267/+;
ash2112411/ash2112411wings show blisters with extra
vein tissue (B) and reduction of interveins B and D alongside partial fusion
of L2-L3 and L4-L5 (C). (D) Detail of extra crossveins connecting L2 and L3.
(E) rho expression in a bs03267/+;
ash2112411/ash2112411 pupal wing. (F) High
magnification of the same image. (G) Bs staining in a wild-type third instar
wing disc. The vein territories and the DV boundary are devoid of antibody
staining, which is only associated with intervein territories. (H)
ash2I1 clones in a wing imaginal disc (visualised as black
spots by the absence of GFP). (I) Bs staining of the same disc. The reduction
of Bs staining is more pronounced in the clone located between L2 and L3
(arrow) than in the one between L3 and L4 (arrowhead). (J) Merged green and
red channels. (K-M) 24-30 hours APF wings stained with anti-Bs. (K) Wild type.
(L) ash2112411/ash2112411 where extra vein
tissue is restricted to wider p-cv and an extra proximal crossvein (arrow).
(M) ash2112411/ash2112411 pupal wing of an
individual with thicker vein tissues. In both L and M, some interveins are
reduced and show some negatively stained cells within the interveins. (N)
ash2I1 mitotic clones in pupal wings. (O) Bs staining of
the same wing. (P) Merged green and red channels. (Q-S) High magnification of
an ash2I1 clone.
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