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Fig. 2. p63–/– prostate lacks basal cells. Both
p63+/+ (A) and p63–/– (B)
urogenital sinus (UGS) formed prostate when grafted into intact male hosts.
When p63–/– UGS was grafted into castrated
male hosts, the UGS developed into a large cyst without ducts (C). The
prostatic ducts were surrounded by smooth muscle cells expressing smooth
muscle actin (SMA) (D,E). Morphologically definable basal cells (black arrow
in F,H) were not observed in p63–/– prostate
(G,I). p63–/– prostate contained goblet cells
(G,I, white arrows) (see also Fig.
3). Elongated/flat nuclei of stromal cells should not be confused
with basal cells. Expression of basal cell markers was examined in
p63+/+ (J,L,N,P) and
p63–/– (K,M,O,Q) prostates. p63 (J,K), K14
(L,M), transglutaminase II (TGII,N,O) and maspin (P,Q) were detected only in
p63+/+ prostate. All basal cell markers were absent in
p63–/– prostate (K,M,O,Q). RT-PCR for K14 (R)
confirmed results of IHC. K14 mRNA was detected in host prostate (coagulating
gland + ventral prostate + dorsolateral prostate, lane 1) and three
p63+/+ prostatic grafts (lanes 2-4) but not in
p63–/– prostates (lanes 5 and 6). ß-actin
was used for control.
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