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Fig. 5. Effect of testosterone. One month after castration (castrated A and B),
proliferation markers, phospho-histone H3 (pH3; a,d,g,j), phospho-MEK (pMEK;
b,e,h,k) and Cdk4 (c,f,i,l) were very low to undetectable in both
p63+/+ (A) and p63–/– (B)
prostates. Five days after implantation of a 25 mg T-pellet (castrated+T;
C,D), all three proliferation markers were upregulated in both
p63+/+ (C) and p63–/– (D)
prostates. In the p63–/– prostatic grafts,
small bud-like outgrowths were visible (D, red arrows). (E) Labeling index for
pH3. Data was analyzed with Fisher's PSLD and ANOVA factorial test. Data was
indicated as mean+s.d. The bars with asterisk are significantly higher than
others (P<0.05). There's no significant difference among bars
without asterisk. Both p63+/+ and
p63–/– prostates were proliferation-quiescent
in the intact and castrated hosts. T-treatment of castrated hosts increased
pH3-positive cells equally in luminal cells of p63+/+ and
p63–/– prostates (+T 5 days). However, luminal
cells in both p63+/+ and
p63–/– prostates became
proliferation-quiescent one month after T-pellet implantation (+T one month).
One month after T-pellet implantation, regenerated
p63–/– prostate expressed markers specific for
prostatic luminal epithelium [androgen receptor (AR) (F), and secretory
proteins, mouse dorsolateral prostate (mDLP) (G) and mouse ventral prostate
(mVP) (H)].
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