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Fig. 2. AtBRM is a nuclear protein associated with a high molecular mass complex.
(A) Nuclear and cytoplasmic proteins from inflorescence apices were subjected
to western blotting using two different antisera against two different regions
of AtBRM (
-AtBRMa and
-AtBRMb). (B) Gel filtration analysis of
nuclear extracts of Arabidopsis calli. Fractions from chromatography
through a Superose 6 HR column were separated by SDS-PAGE and examined by
western blotting using
-AtBRMb antiserum. Fraction numbers are given
across the top and arrows indicate elution position of molecular mass
standards.