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Fig. 4. Gene expression patterns in embryos injected with MO1. (A,B) Expression of
Goosecoid is downregulated in response to MO1 (B), but its expression
domain is unaltered. (C,D) Downregulation of Xbra in response to MO1
(D). See text for details. Embryos in A-D were fixed at similar morphological
stages rather than at different times after fertilisation to compensate for
slight delays in development. (E-H) The expression domains of Xvent-1
(E,F) and Xwnt-8 (G,H) are unaffected by MO1, even though their
expression levels are very slightly (Xwnt-8;
Fig. 3) or significantly
(Xvent-1; Fig. 6)
elevated. Embryos were injected at the two-cell stage, with 20 ng MO1 into
each blastomere.
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