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Fig. 7. Inhibition of activin function causes an upregulation of Xnr2
expression and a downregulation of Derrière. (A) Embryos were
injected with the indicated antisense morpholino oligonucleotides and allowed
to develop to early gastrula stage 10.5, when they were assayed for expression
of nodal-related genes and Derrière by real-time RT
PCR. (B-G) Upregulation of Xnr2 expression is not accompanied by an
expansion of its expression domain. Embryos were subjected to in situ
hybridisation to detect Xnr2 RNA and viewed from the vegetal pole
(B-D) or from the side (E-G). Transcription of Xnr2 in embryos
injected with MO1 (C,D) or MO3 (F,G) is not observed beyond its normal
expression domain. (H-J) Downregulation of Derrière is not
accompanied by a restriction in its normal expression domain. All embryos were
injected at the two-cell stage, with 20 ng MO1 or 15 ng MO3 into each
blastomere.
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