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Fig. 1. Screening for potential PKA substrates from a mouse oocyte library. (A) Radiolabeled protein pools were generated and analyzed by a SDS-gel mobility shift assay. Asterisks indicate the proteins where mobility was shifted by incubation with PKA. (B) Radiolabeled proteins derived from isolated single clones (1-4B, 1-5B, 3-7F and 14-12D) were incubated with or without the PKA catalytic subunit, followed by a SDS-gel mobility shift assay. (C) mRNA from each independent clone were synthesized in vitro. Twenty nanograms of each mRNA (in 20 nl of H2O) or 20 nl of H2O (vehicle) were injected into 30 Xenopus oocytes 12 hours before 500 nM progesterone stimulation. Percentage of GVBD was measured by counting white spots on the animal pole of Xenopus oocytes.





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