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Fig. 7. Effect of phosphatase-dead C-PTPN13 injection on Xenopus oocyte maturation. (A) Schematic diagram of full-length mouse PTPN13 (mPTPN13), HA-tagged C-terminus mPTPN13 (C-PTPN13 or C-PTPN13 WT) and HA-tagged C-terminus C2374S mutant mPTPN13 (C-PTPN13 PD), a construct where the conserved cysteine 2374 was mutated to serine. (B) mRNA coding for a HA-tagged C-PTPN13 (C-PTPN13; C-PTPN13 PD) or vehicle (H2O) was injected into Xenopus oocytes. Twelve hours later, oocyte extracts were prepared and the expression of the two constructs was detected using HA antibodies. (C) Twelve hours after injection of these mRNAs, oocytes were treated with 500 nM progesterone and resumption of meiosis was scored at different times of incubation. Results shown are representative of three independent experiments.





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