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Fig. 9. Effects of PTPN13 siRNA injection on progesterone-dependent changes in
signaling molecules in Xenopus oocytes. Ten nanograms of PTPN13
siRNA-1 or scrambled siRNA-1 were injected into Xenopus oocytes.
After 12 hours, oocytes were stimulated with 500 nM progesterone for 8 hours.
The oocyte lysates were subjected to 8% SDS-PAGE, and the expression and
phosphorylation of each protein were detected by western blotting
analysis.