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Fig. 4. Scanning electron microscopy reveals defects in monocilium formation in
mD2LIC–/– mouse embryos. (A) The distal tip of
a wild-type embryo at 7.5-8.0 dpc (1-2 somite stage) viewed at low power.
(C,E) The gross morphology of the node of homozygous mutant embryos is normal.
Conditions and magnification are identical in A and C, and E is at twice the
magnification. (B,G) Higher-power view of a wild-type embryo reveals rounded
cells bearing monocilia (arrowheads). (D,H,F,I) Ventral node cells in
mD2LIC–/– embryos are flatter than their
wild-type counterparts and they lack normal monocilia. In some cases stunted
structures are formed in the place of monocilia (arrows). G-I are at the same
magnification. White boxes in B,D,F indicate regions of the node shown at
higher magnification in G-I respectively. (F) Cells in the anterior region of
the node and notochordal plate have the most extreme phenotype.
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