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Fig. 5. Analysis of the mD2LIC phenotype at gastrula stages. (A,B)
Expression of Fgf8 is unaffected by loss of mD2LIC function.
(A) Lateral view of wild-type embryo; (B) Lateral view of
mD2LIC–/– embryo. (C-E) Shh
expression is reduced in the node and axial mesoderm of mD2LIC-null
mutants. (C) Wild-type embryo showing expression of Shh in the node
and in axial structures. (D,E) Reduction of Shh expression
(arrowheads) in mD2LIC–/– embryos. (F-J)
Reduction in Foxa2 expression in mD2LIC null mutant embryos. (F)
Wild-type embryo showing expression of Foxa2 in the node and axial
mesendoderm. (G,H) Expression of Foxa2 is severely reduced (G) or
absent (H) in mD2LIC-null mutant embryos. (I,J) Foxa2
expression is later reduced in anterior definitive endoderm in
mD2LIC–/– embryos. (I) Wild-type embryo. (J)
mD2LIC–/– embryo. Arrowheads mark anterior
definitive endoderm. (K-P) TUNEL analysis of
mD2LIC–/– embryos reveals no elevation of cell
death in the node or its derivatives but apoptosis does occur in anterior
definitive endoderm. (K-M) Little apoptosis is observed in the embryonic
region of wild-type embryos at 7.5 dpc (K, lateral view; L, anterior view; M,
distal view). In mD2LIC–/– embryos, no
apoptosis is observed in the node or its derivatives (P) but substantial cell
death occurs in the anterior definitive endoderm (arrowheads, N, lateral; O,
anterior).
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