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Fig. 1. Foxg1Cre removes the Hedgehog responsiveness of
telencephalic progenitors in conditional Smo mice. (A-C) ß-gal
expression of Foxg1Cre;ROSA26R mice shows the extent of
Cre-mediated recombination at E7.5 (A), 10-16 somite stage (B), and E9.5 (C).
Note that A is a flattened dissection of the anterior neural plate as
diagrammed in the inset, with the anterior neural ridge at the top and the
broken line representing the approximate telencephalic primordium. The arrow
in B is marking the lacZ staining in the anterior neural ridge. (C)
Foxg1Cre-mediated recombination extends throughout the
anterior-posterior extent of the E9.5 telencephalon. (D,E) Coronal section of
an E10.5 Smoc/– (D) and
Smoc/–;Foxg1Cre mutant (E) telencephalon
showing the absence of Gli1 transcripts in the latter, a sensitive
readout of active Hedgehog signaling. (F) Comparison of E18.5 wild-type and
Smoc/–;Foxg1Cre mutant embryos. (G)
Wholemount dorsal view of wild-type and
Smoc/–;Foxg1Cre mutant brains shows
severe size reduction of the telencephalic vesicles and olfactory bulbs. The
rudimentary olfactory bulb of the mutants is marked with an asterisk. Scale
bars: 100 µm in A; 200 µm in D,E.
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