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Fig. 5. Most cortical interneuron precursors are dependent on early Hedgehog signaling. (A-F) Coronal sections of an E12.5 wild-type (A,C,E) and Smo^c/–;Foxg1^Cre mutant (B,D,F) telencephalon, stained by in situ hybridization. The Dlx2, Lhx6 and GAD67 genes have been implicated in various aspects of interneuron development, and are expressed strongly in the basal E12.5 telencephalon (A,C,E). These genes are absent from the Smo^c/–;Foxg1^Cre mutant telencephalon (B,D,F). (G-J) Coronal sections of an E18.5 wild-type (G,I) and Smo^c/–;Foxg1^Cre mutant telencephalon (H,J), stained with antibodies against Calbindin and GABA. Both Calbindin and GABA are expressed by cortical interneurons throughout the layers of the wild-type cortical plate (G,I, see insets). In Smo^c/–;Foxg1^Cre mutants, Calbindin and GABA staining is absent from the cortical plate and is restricted to a ventral-lateral region of the cortex (H,J, see insets). Scale bars: 300 µm in A-F; 500 µm in G-J for low-power field, and 125 µm for high-power inset.

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