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Fig. 1. Tbx2 expression during cardiogenesis, 8.5-9.5 dpc. (A) Right view
of an 8.5 dpc embryo showing Tbx2 expression in the allantois (al)
and cardiac crescent (cc). The line indicates the plane of the section shown
in B. (B) Vibratome section of an 8.5 dpc embryo stained in whole-mount,
showing expression in cardiac mesoderm (cm). (C) Ventral view of the head and
heart of an 8.5 dpc embryo, showing Tbx2 expression in the otic
placode (op), inflow tract (ift) and septum transversum (st). (D) Right view
of a 9.5 dpc embryo showing previously unreported Tbx2 expression in
somites (s) and nephrogenic mesoderm (nm). The line in the trunk indicates the
plane of section in G. (E) Left view of the embryo shown in D. Labeled are the
dorsal retina (dr), otic vesicle (ov), forelimb bud (fl) and previously
unreported expression in the body wall of the caudal trunk and tail (bw). The
line in the tail region indicates the plane of the section in F. (F) Vibratome
section of a 9.5 dpc embryo showing specific expression in the nephrogenic
mesoderm (nm). (G) Vibratome section of a 9.5 dpc embryo showing expression in
pharyngeal arch mesenchyme (arrow), also the site of cardiac neural crest
cells migrating into the outflow tract (oft). Note the absence of expression
in the aorticopulmonary septum (arrowhead). (H) Right whole-mount view of a
9.5 dpc heart showing Tbx2 expression in the outflow tract (oft). (I)
Left view of the heart shown in G, demonstrating Tbx2 expression in
the atrioventricular canal (AVC) and septum transversum. (J) Ventral view of a
dissected 9.5 dpc heart stained in whole-mount, showing Tbx2
expression in the outflow tract (oft). (K) Ventral view of the heart in J with
the outflow tract removed, showing Tbx2 expression on the outer
curvature of the AVC (arrowhead). (L) Dorsal view showing Tbx2
expression in the AVC and ventricular inner curvature (arrow). nf, neural
fold; c, coelom; fg, foregut; nt, neural tube; da, dorsal aorta; hg, hindgut;
rv, right ventricle; v, ventricle; a, atrium.
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