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Fig. 7. Reduction of Pebble function in wings by RNA interference causes cytokinetic failure. (A,C,E) Low magnification images of whole adult wings. (A',C',E') Corresponding high magnification images of the posterior region of the wing (framed in A) that was used to evaluate phenotypes (see Fig. 8E). (A,A') A wild-type wing showing normal size and shape of the wing (A) and ordered arrangement of hairs (A'). (B,B') A high magnification of a wild-type pupal wing stained with Phalloidin to detect F-actin (red) and Hoechst 33258 for DNA (blue). Each individual wild-type cell has one nucleus (B) and one prehair F-actin bundle (B'). (C,C') An en-GAL4>UAS-pbl^RNAi wing showing diminution of Engrailed-domain of the wing (C) and wing hair phenotype (C') resulting from reduced pbl activity. Many en-GAL4>UAS-pbl^RNAi adult wing cells have more that one hair. (D,D') A high magnification view of an en-GAL4> UAS-pbl^RNAi mutant pupal wing stained with Phalloidin for F-actin (red) and Hoechst 33258 for DNA (blue). Mutant cells show more than one nucleus (D) and prehair F-actin bundles (D'). (E,E') Loss of one copy of RhoA^72R further reduces the Engrailed-specific region of the en-GAL4>UAS-pbl^RNAi wing (E) and increases the number of multihaired cells (E'). The multiple haired cell phenotype is quantified in Fig. 8E.

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