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Fig. 6. PGCs proliferate with random divisional orientation. (A-D) Late third
instar larval ovaries containing dividing PGCs (outlined by thick dotted
lines). The ovaries were double stained with anti-Vasa antibody (green) to
label all PGCs, and anti-1B1 antibody (red) to visualize spectrosome
orientation in PGCs and to outline somatic cells. Dividing PGCs in contact
with TF/CpCs (outlined by thin dotted lines) are oriented perpendicularly
(A,C) or in parallel (B), or approximately 45° (D) to the cap cell-PGC
interface. PGCs in contact with posterior somatic cells also divide
perpendicularly (C), or in parallel (A,B), to the soma-germline interface. (E)
A quantitative comparison of the divisional orientation of PGCs contacting
CpCs, of those in the anterior half but not contacting cap cells (anterior
PGCs), and of those in the posterior half (posterior PGCs). The CpC-PGC
interface is assigned a value of 90°. Each red dot in the diagram
represents an individual sample (n=63).
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