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Fig. 7. The fates of EGFP-marked single pole cells in larval and adult testes.
(A,B) A late third instar larval testis derived from an embryonic gonad
containing a labeled anterior pole cell, viewed under low and high
magnifications, respectively. The testis, with its anterior pole to the left,
is stained with anti-1B1 antibody (red) that outlines somatic cells, as well
as spectrosomes and fusomes (F), in germ cells, and with anti-GFP antibody
(green) to identify germ cells derived from the marked pole cell. Note that
GSCs, such as the one marked by an arrow in B, are in direct contact with the
hub (H; arrowhead), and have produced multiple germline cysts. (C) EGFP image
of an adult testis showing a similar marking of GSCs (arrow) and their
progeny. (D,E) A late third instar larval testis derived from an embryonic
gonad containing a labeled posterior pole cell, viewed under low and high
magnifications, respectively. The testis, with its anterior pole to the left,
is stained with anti-1B1 (red) and anti-GFP (green) antibodies. Note that no
GFP-labeled germ cells are near the hub (H). (F) EGFP image of an adult testis
showing that the GSC position (white thin arrow) is not marked, while a
two-cell germline cyst (white thick arrow) is marked by EGFP, together with
several other more advanced cysts. Scale bars: in A, 100 µm for A,B,D,E; in
C, 500 µm for C,F.
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