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Fig. 10. Physical interactions between Wnt3A and its palmitoylation site mutant with the extracellular domain of LRP6 and mouse Fz8. Conditioned medium of Rat2 cells expressing HA-tagged Wnt3A or Wnt3A C77S was mixed with conditioned medium of 293 cells expressing LDLR-IgG (A,B), LRP6N-IgG (A), mFz8CRD-IgG (B), and incubated protein G-agarose beads. Precipitates were washed and fractionated by SDS-PAGE, and immunoblotted with anti-HA antibodies (upper panel). Input of Wnt3A in this pull down assay was determined by immunoblotting with anti-HA antibodies (lower panel).





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