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Fig. 1. The reeler phenotype is preserved in slice cultures of
hippocampus. (A) Slice culture of wild-type hippocampus, prepared on P0 and
incubated for 7 days in vitro (DIV). Staining for NeuN reveals dense packing
of pyramidal neurons in CA1 and CA3, and of granule cells in the granular
layer (g) of the dentate gyrus (DG). (B) Slice culture of reeler
hippocampus, prepared on P0 and incubated for 7 DIV. NeuN-stained pyramidal
neurons and granule cells show the migration defect characteristic of the
reeler hippocampus. Pyramidal neurons in CA1 form a double layer
(asterisks), and the granule cells are scattered all over the dentate gyrus.
(C) Double-labeling for NeuN (red) and GFAP (green) in a slice culture of
wild-type dentate gyrus. Long GFAP-positive radial glial fibers run
perpendicular to the granular layer. g, granular layer; h, hilus; m, molecular
layer. (D) Detail of reeler dentate gyrus double-labeled for NeuN and
GFAP. Granule cells do not form a circumscribed layer, and GFAP-positive cells
have short processes, thus resembling typical astrocytes. Scale bars: 100
µm in A,B; 20 µm in C,D.
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