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Fig. 6. Western blots for reelin under the different experimental conditions. Lane
1, supernatant of reelin-transfected 293 cells. Arrowheads indicate the
full-length protein and its characteristic fragments. Lane 2, supernatant of
control 293 cells. Lane 3, freshly prepared culture incubation medium. Lane 4,
freshly prepared supernatant (200 µl) from reelin-transfected 293 cells,
added to 800 µl incubation medium (as was used in the experiments with
reeler cultures). Lane 5, lysate of rat hippocampus (P5). Lane 6,
lysate of slice cultures of P5 rat hippocampus incubated in vitro for 7 days.
Lane 7, lysate of slice cultures of P0 reeler hippocampus incubated
in the presence of reelin (DIV 7). Lane 8, same incubation medium as in lane
4, after 2 days of incubation. Lane 9, incubation medium from wild-type slice
cultures after 2 days of incubation.
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