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Fig. 8. Rescue of granule cell orientation and layer-specific commissural input.
(A,B) Scattered distribution of biocytin-labeled granule cells in the dentate
gyrus of a reeler hippocampal slice culture. Granule cell dendrites
and axons (red in B) extend in all directions. In A, neuronal somata were
counterstained for Cresyl Violet to show the loose distribution of granule
cells. (C,D) Many biocytin-labeled granule cells in the rescued granular layer
of a reeler slice culture show normal dendritic and axonal
orientation. Neuronal somata are counterstained for Cresyl Violet (C) to
illustrate the formation of a granular layer in the reeler culture.
Dashed line represents border between cultures; the boxed area is depicted in
D. (E,F) The laminated projection of commissural fibers to the reeler
dentate gyrus is only rescued when a compact granule cell layer has formed.
(E) Co-culture of rat dentate gyrus and P4 reeler dentate gyrus.
`Commissural' fibers from the rat dentate gyrus are scattered all over the
reeler dentate gyrus, like their target granule cells, counterstained
for Cresyl Violet. (F) By contrast, when a rat dentate gyrus is co-cultured
with P0 reeler dentate gyrus, a granular layer and a compact
`commissural' projection (arrowheads) have formed. Dashed lines represent
borders between cultures. Scale bars: 45 µm in A,B; 50 µm in C; 30 µm
in D; 70 µm in E; 50 µm in F.
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