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Files in this Data Supplement:
Fig. S1. Glial cell number is not controlled by apoptosis. Confocal projections of multiple labelling: TUNEL (green), anti-Repo (red) and anti-Elav (blue) on wild-type wings at 20 hours (A), 22 hours (B), 26 hours (C), 30 hours (D) and 36 hours (E) APF. In panel A, yellow nucleus indicated by arrowhead is not due to the co-localisation of Repo and TUNEL labelling, as confirmed by the analyses of optical sections. Symbols are as in Fig. 1. Scale bar: 40 mm.
Movie 1. Glial proliferation and directional migration in the wing. Time-lapse showing repo-ncGFP (nuclear and cytoplasmic GFP) labelling in the whole wing from 19 hours to 26 hours APF. L1 and L3 glial cells migrate proximally and meet glia differentiating in the radius vein. Time-lapse show the simultaneous occurrence of migration and proliferation along the L3 nerve.
Movie 2. Fast cytoskeleton reorganisation in wing glia. Time-lapse realised on an isolated repo-actinGFP GPI on L3. The cell displays numerous thin actin-containing processes (filopodia) that grow and retract in the range of minutes.
Movie 3. Front of migration on L1. Time-lapse (17 hours to 19 hours 40 minutes APF) on the whole glial population at the L1 front of migration. Pioneer cells display very long and dynamic filopodia compared to follower, more distal, cells.
Movie 4. Glia-glia interactions control cell migration. Movie on L3 glia showing that cells belonging to one lineage move until they reach more proximal glial cells. Glial cells from different sensory organs tend to not intermingle, as distally located glia do not bypass proximal glia. Notice that cells from different lineages divide at the most with one-hour difference.
Movie 5. Mis-oriented migration of supernumerary glia at posterior margin of Hw49c wings. Time-lapse analysis in an Hw49c; repo-ncGFP mutant wing displaying two ectopic glia on the posterior margin. The distal glial cell migrates proximally, the proximal one divides and migrates distally.
Movie 6. Glial proliferation followed by time-lapse. Time-lapse on repo-nlsGFP glial cells. This movie allows us to follow L3 lineages from 23 hours to 33 hours APF with a thirteen minutes interval. L3.3 glia is artificially coloured in red, L3.1 glia in green and L3.v glia in blue. Notice the absence of glial cell death.
Movie 7. GPIId ablation. L3.3 lineage followed for several hours by time lapse after killing the distal GPII. The cell targeted for ablation is indicated by the purple arrowhead. The remaining GPII divides once.
Movie 8. GPIIp ablation. L3.3 lineage followed for several hours by time lapse after killing the proximal GPII. The cell targeted for ablation is indicated by the purple arrowhead. The remaining GPII divides once.
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