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Fig. 10. Ablation within a glial lineage. (A,B) Examples of GPIId (A) and GPIIp (B)
ablations performed at 22 hours APF. Symbols are as in
Fig. 5. (a) Schematic
representation of the ablation. (b-d) GFP labelling before (b), just after (c)
and several hours (d) after ablation. Asterisks in panel c show fading of the
GFP labelling in the targeted cell. Notice (d) that the spared GPII remains
labelled by GFP and undergoes division. (e) Immunolabelling at 32 hours and 30
minutes APF, using anti-GFP and anti-Elav. Brackets indicate the remaining
glia progeny of L3.3 and L3.1 GPII. L3.2 indicates the L3.2 neurone. (C)
Average number of glial cells at 32 hours and 30 minutes APF in a wild-type
(WT) wing, and in GPIIp or GPIId ablated wings. Glial cells from each L3.3
sublineage were followed by time-lapse; n indicates the number of
samples analysed. Bars indicate highest and lowest values. Scale bars: 40
µm.
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