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Fig. 2. Glial development followed by time-lapse. (A) Pupa preparation for time-lapse. Red lines indicate the path of puparium case dissection. The operculum is opened (left panel, straight line), and the puparium case over the wing is removed (mid and right panel, dashed lines). Left and mid panels are dorsal views; right panel is a lateral view. Anterior is to the top. W, wing. (B) repo-actinGFP: time-lapse images of a GPI showing dynamic cell shape and filopodia reorganisation (arrowheads). (C) Time-lapse images showing repo-ncGFP (nuclear and cytoplasmic GFP) labelling (see Movie 1 in supplementary material). Symbols as in Fig. 1B. Brackets indicate the glial cells originating from L3 gliogenic sensory organs. Arrows show the L1 front of migration. Arrowheads indicate the positions of L3.3 glial nuclei. Scale bars: 20 µm (B); 70 µm (C).





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