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Fig. 4. Confocal-assisted cell ablation. (A) Ablation of all L3 glial lineages (L3.3, L3.1 and L3.v) at 17 hours APF on a repo-ncGFP wing. GFP labelling before (upper panel) and just after (lower panel) UV laser irradiation. Asterisks indicate the position of the ablated cells. (B) Immunolabelling on the same wing five hours after ablation. Notice the absence of specific Repo (upper and lower panels) and GFP (upper panel) labelling on L3 (bracket). (C) A wild-type wing at a comparable stage, labelled with anti-Repo. + indicates unspecific labelling corresponding to the wing cuticle. Scale bars: 40 µm.





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