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Fig. 5. Glia-glia contact inhibit migration. In all schematic drawings (A, panels a-c; B, panels a-d), distal glia are shown in light green and proximal/medial glia in dark green. Glial cells are represented by circles, neurones by red squares, the laser beam is indicated in purple. (A) Glia ablations on 17 hours APF repo-ncGFP wings. (a-c) Ablation of proximal/medial (L3.1 and L3.v; P+M), bilateral (L3.3 and L3.v; P+D) and distal (L3.3; D) glial cells, respectively. (d-i) GFP labelling before (d-f) and just after (g-i) ablation. Asterisks indicate the positions of the ablated cells. (B) Same wings as in A, 15 hours after cell ablation. (a-d) Schematic representations of the results. (e-l) Immunolabelling with anti-GFP (green), anti-22c10 (red; neuronal somata and axons) and anti-Elav (blue; neuronal nuclei). Symbols are as above. (e-h) anti-GFP. (i-l) Multiple labelling. Scale bars: in A, panels d-i, 40 µm; in B, panels e-l, 70 µm.





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