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Fig. 6. Glia migration in the absence of neurones. (A-C,E,F) N^ts1: N^ts1; repo-ncGFP wings after complete transformation of the L3.3 gliogenic lineage into glia immunolabelled at 13 hours (A,B), 20 hours (C) and 24 hours (F) APF. (B) Triple labelling with anti-Repo (red), anti-Elav (blue) and anti-Cut (all SOP progeny, green). (C,D) Wings labelled with anti-Elav (red) and anti-GFP (green). (E-G) Wings immunolabelled with anti-22c10 (red) and anti-GFP (green). A corresponds to the blue channel of B, and E corresponds to the red channel of F. Notice the absence of the L3.3 neurone (arrows) in the N^ts1 wings, as shown by lack of neuronal labelling (anti-Elav in A-C; anti-22c10 in E,F). (D,G) Wild-type (WT): (repo-ncGFP) wings at 20 hours (D) and 24 hours (G) APF. In all panels, brackets indicate L3.3 glia. Symbols are as above. Scale bars: 40 µm (A-D); 70 µm (E-G).

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