DEV01300 Supplementary Material

Files in this Data Supplement:

• Table 1 (PDF)
• Table 2 (PDF)
• Table 3 (PDF)
• Supplemental Figure 1A-F
• Supplemental Figure 1G-L
• Supplemental Figure 1M-R -

  Fig. S1. In situ hybridization analysis of genes affected in or^J mutants. (A-R) Sections through E14.5 or E17.5 or^J/+ heterozygous or or^J/or^J homozygous littermates, as indicated, were analyzed for the following RNAs: (A) Notch1, (B) E2f1, (C) cyclin E1, (D) cyclin D1, (E) Neurod1, (F) Math5, (G) Btg2, (H) Dll1, (I) Hes1, (J) Brn3b, (K) Snap25, (L) Stathmin1, (M) Pax6, (N) p57, (O) Otx1, (P) Crhbp, (Q) Igf2, (R) Otx2. Full expression patterns are discussed in the Results. or^J/+ and or^J/or^J sections are at different magnifications. (N,P) Insets show peripheral sections from the same eye. Scale bars: 100 µm.

• Supplemental Figure 2 -

  Fig. S2. In situ hybridization analysis of MITF target genes in or^J mutants. (A-E) Sections through E14.5 (A-D) or E17.5 (E) or^J/+ heterozygous or or^J/or^J homozygous littermates, as indicated, were analyzed for the following RNAs: (A) Trpm1, (B) Dct, (C) tyrosinase, (D,E) Cd63. Full expression patters are discussed in Results. or^J/+ and or^J/or^J sections are at different magnifications. (B) Inset shows peripheral section from the same eye. Scale bars: 100 µm.

• Supplemental Figure 3 -

  Fig. S3. In situ hybridization analysis in MITF^vga-9 mice. (A,B) Sections through E11.5 MITF^vga-9/+ heterozygous or MITF^vga-9/MITF^vga-9 homozygous littermates analyzed for Cd63 RNA (A) or Tfec RNA (B). Tfec RNA was expressed in the RPE and appeared upregulated in Mitf^vga-9/Mitf^vga-9 homozygotes whereas Cd63 RNA, also expressed in the RPE, was reduced but not absent in Mitf^vga-9/Mitf^vga-9 homozygotes. (C-H) Sections through E14.5 Mitf^vga-9/+ heterozygous or Mitf^vga-9/Mitf^vga-9 homozygous littermates analyzed for the following RNAs: (C) Cd63, (D) Tfec, (E) Otx2, (F) p57, (G) Pax6 and (H) Crhbp. Tfec expression was low or absent at E14.5 and unaltered in Mitf^vga-9/Mitf^vga-9 homozygotes. Cd63 was expressed at high levels in the RPE in Mitf^vga-9/+ heterozygotes and wild-type albinos (inset in C), but was absent in Mitf^vga-9/Mitf^vga-9 homozygotes. By contrast, Otx2, p57, Pax6 and Crhbp did not show significantly altered expression patterns in Mitf^vga-9/Mitf^vga-9 homozygotes. Scale bars: 100 µm.