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Fig. 6. Misexpression of Chx10 by in ovo electroporations. (A-U) HH Stage
10 embryos were electroporated with GFP and Chx10 (A,D,G,J,M,P,S) or
GFP alone (C,F,I,L,O,R,U) and allowed to develop an additional day.
(B,E,H,K,N,Q,T) Images are from the left non-electroporated eye of the same
embryo shown in (A,D,G,J,M,P,S) respectively. (A-C) HH16-18 embryos were
harvested and photographed for GFP to observe transfected regions. (D-U)
HH16-18 embryos were harvested and whole-mount in situ hybridization was
performed to detect the following RNAs: (D-F) Mmp115, (G-I)
Mitf, (J-L) Tfec, (M-O) Dct, (P-R) Six3,
(S-T) Chx10 3'UTR, (U) Chx10. (V-Y) Sections
through the Chx10 and GFP electroporated embryo detected for
Mmp115 (D,E). (V) Mmp115 RNA was detected in the RPE of the
eye that was not electroporated (arrow, high magnification image shown in
(X)), but was highly reduced in the eye electroporated with GFP and
Chx10 (arrowhead, high magnification image shown in (Y)). (W) Section
from (V) further stained with an antibody against GFP to show the region of
the embryo that was electroporated (brown stain), including the retina and RPE
in the bottom eye.
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