|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Deletion analysis defines a 220 bp minimal Msx2 element and a 52
bp element required for BMP signaling. (A) Deletion series of lacZ
constructs bearing fragments of the 560 bp promoter sequence. The BMP
responsiveness of each transgene was assessed by bead implantation; positive
response (+), negative response (–). (B,C,E,F,H) lacZ stains of
560 and 220 bp transgenic embryos at E11.5. Expression patterns (B,C) and BMP
responsiveness (E,F) are similar. BMP-responsive constructs shared a 52 bp
region (A, *), deletion of which in the context of the 560 bp
transgene resulted in reduced expression (D), and loss of BMP responsiveness
in embryos (G) and in 10T1/2 cells (I). Because of diminished transgene
activity, the embryo in D was stained overnight (compare with H), while
embryos in B and C were stained for 1 (B) and 2 (C) hours.
|
