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Fig. 7. The Msx2 BMPRE accurately interprets Dpp signals in
Drosophila. (A-D) Msx2 transgenes behave like Dpp-responsive
genes in imaginal discs. In situ hybridization showing dpp expression
(A). Expression of a vestigial-lacZ reporter gene, a known
dpp target (B). Expression of the 480bpMsx2-lacZ transgene
(C). The 480 bp fragment is the conserved region identified in
Fig. 2,
Fig. 3A. Expression of the
480bpMsx2-lacZ transgene in the wing pouch with ectopic activation of
the Dpp pathway by A9Gal4>TkvA (D). (E-I) dpp and
220bpMsx2-lacZ transgene expression in stage 13 embryos (lateral
view). dpp in situ hybridization showing wild-type expression pattern
in parasegments (ps) 3 and 7 of the embryonic mid-gut (E) and in dpp
(S11/S22) mutant (F), in which dpp expression is lost in ps3
(arrow), but not in ps7. 220bpMsx2-lacZ transgene in wild type (G)
and dpp (S11/S22) mutant (H). Expression of 220bpMsx2-lacZ
transgene throughout the gut in response to ectopic expression of Dpp driven
by a heat-shock promoter (I). (J-Q) dpp and 52bpMsx2-lacZ
transgene expression in early blastoderm embryos (lateral view). dpp
in situ of a stage 5 embryo showing restriction of dpp expression to
the dorsal part of the embryo (J). Expression of the 52bpMsx2-lacZ
transgene in wild-type (K) and dorsal mutant (L) embryos. Expression
of 52bpMsx2-lacZ transgene in screw (M) and brinker
(O) mutant embryos, and in an embryo with ectopic activation of Dpp using
Tub Gal4>UAS Dpp (N). 52bpMsx2-lacZ transgene expression
is lost when the Smad sites (P) or homeodomain site (Q) are mutated.
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