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Fig. 1. Features of the Neurotactin-positive bundles in the thoracic CNS of larval
Drosophila. (A) A thick transverse projection of the bundles in one
thoracic neuromere. Brackets show the levels of the dorsal (D), intermediate
(I) and ventral (V) commissures; VA, ventral arch; tinted region highlights
the right ventrolateral (VL) neuropil. (B) Schematic representation of a cross
section through the neuropil, showing positions of the commissures,
ventrolateral neuropil and dorsal longitudinal tracts (DT). (C,D) Dorsal views
of confocal sections through the VL neuropils in T2 and T3 of a nervous system
immunostained for (left) Neurotactin (magenta) and with phalloidin (green).
Phalloidin staining shows the fine fibrous neuropil characteristic of the VL
neuropil, which contains a poorly stained core, the lateral cylinder (LC),
that is bounded by the Neurotactin bundles 9i and 1c (right). Several bundles
ascend through the cylinder. (E-I) Paired images of thick stack projections of
the T2 and T3 neuropils from the levels indicated on (A). The lineage bundles
are labeled on the negative version (right panel); the next ventral section is
also included as a `ghost' to aid in alignment of the bundles. Brackets show
the anterior (aD) and posterior (pD) dorsal commissures, the anterior (aI) and
posterior (pI) intermediate commissures, and the anterior ventral (aV)
commissure.
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