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Corrigendum for Li et al., Development 130 (13) 2819-2827.
First published online September 30, 2004

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Scabrous and Gp150 are endosomal proteins that regulate Notch activity

Yanxia Li, Michael Fetchko, Zhi-Chun Lai and Nicholas E. Baker Development 130, 2819-2827.

Figure 5 of this paper illustrates the distribution of Sca protein and of several deletions of Sca after heatshock-induced expression in the pupal retina of Drosophila. Owing to confusion between two plasmids in N.E.B.'s laboratory, the preparation labelled Sca41-514 in Fig. 5G was, in fact, expressing a different protein, Sca319-463. When the experiment was repeated using the correct genotypes, we found that whereas full-length Sca associated with the lattice of pigment cells that express the Notch protein at high levels, Sca41-514 associated predominantly with other cells. The results indicate that the N-terminal 514 amino acids of Sca mediate colocalization with Notch, not the Fibrinogen-Related Domain contained within amino acids 515-774. We apologize to readers for this mistake, which does not affect the major conclusions of the paper. A revised version of Fig. 5 and its legend are shown below.

View larger version (103K): [in this window] [in a new window]   Fig. 5. Sca and N association in pupal retina. Immunohistochemistry was used to label proteins in pupal retinas. (A,E) N protein in wild type (A) or in gp1503/gp1504 (E). (B-D,F-H) Sca protein. (B) Sca is absent from wild-type retinas until weak expression begins in a single sensory organ lineage between each ommatidium. (C) Twenty minutes after heatshock-induced expression, intracellular Sca protein is distributed homogenously. (D) Sixty minutes after heatshock-induced expression, Sca protein is concentrated in particles within the pigment cell lattice that also expresses N (compare with A). (F) Sixty minutes after heatshock-induced expression, Sca protein is not concentrated in particular cells from gp1503/gp1504 retinas. (G) Sixty minutes after heatshock-induced expression, Sca41-514 is concentrated in cells that lack N expression, the converse of the pattern seen with full-length Sca (compare with D). (H) Sixty minutes after heatshock-induced expression, Sca513-773 shows some concentration in N-expressing cells, although reduced compared with that of full-length Sca (compare with D).

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This Article Figures Only Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?