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Fig. 10. Nkx6 misexpression affects both motoneurons and interneurons in
flies. (A) Wild-type fly embryo with normal numbers of aCC, pCC, and RP2
(arrow) neurons (n=48). (B) In sca>Nkx6 (fly gene)
embryos RP2 is duplicated (arrow) in 75% of hemisegments (n=34). (C)
Wild-type embryos have 5.0±0.14 U motoneurons (asterisks;
n=48) and 10.0±0.73 EL interneurons (circle; n=46).
(D) In sca>Nkx6 embryos both types of neurons are reduced (Us,
2.5±1.4, n=34; ELs, 5±3.4, n=34). (E) In wild
types there are 3.8±0.48 RP1,3,4,5 motoneurons (circle; n=80)
and 2.8±0.37 EW interneurons (arrowhead; n=25). (F) In
sca>Nkx6 (fly gene overexpression, OE) RP1,3,4,5 are normal
(circle; 3.6±0.65; n=73) and EWs are reduced (arrowhead;
1.0±1.2; n=30). In some cases, as shown here, an EW-like cell
ectopically expresses pMAD, indicating an interneuron-to-motorneuron fate
change; these cells were not scored as EWs. (G) In sca>nkx6.1
(fish gene) RP1,3,4,5 numbers are normal (circle, 3.5±0.66;
n=55). (H) Wild-type embryos have one medial lateral motoneuron (ML)
per hemisegment (asterisk; 80±0; n=80) and 4.7±0.54
HB9-, Islet-, pMAD-positive lateral cluster motoneurons (LC; circle;
n=50). (I) sca>Nkx6 (fly gene) embryos have one ML
motoneuron (asterisk; 94% of hemisegments; n=44) and 8.5±2.3
LC motoneurons (n=80). (J) sca>nkx6.1 (fish gene) also
have one ML motoneuron (asterisk, 89.4% of hemisegments; n=57) and
8.0±1.89 LC motoneurons; (n=60). (K) Schematic depicting
average wild-type number of assayed motoneurons and interneurons. (L)
Schematic depicting average number of motoneurons and interneurons in
sca>Nkx6 (fly gene) embryos. Scale bar: 15 µm. All images are
ventral views; rostral is up, medial is left and lateral is right.
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