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Fig. 5. Nkx6 is expressed in fly ventral CNS and motoneurons. (A) Ventral
view of stage 9 embryo showing Nkx6 RNA in cephalic neurectoderm and
CNS midline. (B) At stage 10, Nkx6 is ventrally restricted to rostral
neuroblasts in each hemisegment. (C) By the end of gastrulation Nkx6
is expressed in neurons throughout the CNS. (D-F) Nkx6 is expressed
in neuroblasts 1-1, 1-2, 2-2, 3-1, 3-2 and 4-2. Neuroblasts expressing
Nkx6 mRNA (blue) were identified by their position relative to
Eagle-positive neuroblasts (brown; NB2-4, arrowhead; NB3-3, arrow; NB6-4,
asterisk; NB7-3, white arrowhead) and other neuroblasts. The same two segments
of a stage 11 embryo are shown at different focal planes (D, superficial; E,
middle; F, deep). White line, ventral midline. (G) At stage 14, Nkx6
(RNA in situ, purple) is confined to a population of neurons distinct from
those expressing the dorsal motoneuron determinant Eve (antibody, brown). The
prominent brown cells near the midline are RP2 motoneurons (*). (H)
pMAD (antibody, brown) and Nkx6 (RNA in situ, purple) expression are
coincident in many cells at stage 14. (I) By stage 16, cells positioned
laterally are positive for pMAD and Nkx6 (bracket), but more medial
cells are clearly only pMAD-positive. Rostral is up in all images. Scale bar:
50 µm for A-C; 16 µm for D-I.
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