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Fig. 6. Hh signaling induces zebrafish nkx6.1 expression. (A) 18 hpf
wild-type expression of nkx6.1. (B) Dramatic dorsal expansion of
nkx6.1 in an 18 hpf embryo ectopically expressing shh
(shh OE, 87% of injected animals, n=283). (C) 12 hpf
wild-type expression of nkx6.1. (D) 12 hpf syu mutant with
reduced nkx6.1 expression. (E) 12 hpf syu mutant injected
with ehh and twhh MOs. nkx6.1 expression is largely
absent (28% of injected clutch from syu heterozygous parents,
n=120). (F) Wild-type expression of nkx6.1 in spinal cord at
24 hpf. (G) 24 hpf syu mutant has wild-type levels of nkx6.1
expression in spinal cord. (H) Few nkx6.1-positive cells remain in a
24 hpf smu mutant spinal cord. (I) 24 hpf syu mutant
injected with ehh and twhh MOs has no nkx6.1
expression in spinal cord (21% of injected clutch from syu
heterozygous parents, n=128). C-D dorsal views; all others are
lateral. Rostral to left in all images. Scale bar: 50 µm for A,B; 100 µm
for C-E; 25 µm for F-I.
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