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Fig. 9. Nkx6 function is conserved between fish and flies. (A)
isl2-positive CaPs and VaPs in an18 hpf zebrafish embryo.
isl2-positive RBs are out of focus. Wild-type embryos have
1.3±0.7 isl2-positive motoneurons per spinal hemisegment
(n=5). (B) 18 hpf embryo ectopically expressing Nkx6 (fly
gene, Nkx6 OE) RNA with supernumerary isl2-positive cells
(2.3±0.3 isl2-positive cells per hemisegment), similar to the
phenotype caused by ectopic expression of zebrafish nkx6.1 RNA
(compare with Fig. 7B). The
number of isl2-positive motoneurons is significantly different
(P<0.0003). (C) PMNs in 24 hpf wild-type embryo labeled with zn1
and znp1; RBs and several axon tracts are also visible. (D) 24 hpf embryo
ectopically expressing Nkx6 (fly gene) RNA generates ectopic PMNs
(compare with Fig. 7D). (E)
Stage 17 wild-type fly embryo stained with Fas II. Arrowheads indicate SNb
nerves. (F) sca>Nkx6.1 (fish gene) fly embryos with
visibly thicker SNb nerves (88%, n=42 hemisegments). (A-D) Lateral
views of zebrafish spinal cord. (E,F) Dissected fly embryo musculature. Scale
bar: 50 µm for A,B; 33 µm for C-F.
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