|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Nkx6 allele generation and expression analysis. (A) Diagram of the
Nkx6 locus, with the location of the P[JG] insertion and the extent
of the D25 deletion indicated. (B) Stage 9, (C) stage 11, and (D) stage 14
wild-type embryos stained for Nkx6. (B) Nkx6 is expressed in midline
precursors at stage 9. (C) At stage 11, Nkx6 is expressed in compact clusters
of neural precursor cells and neurons flanking the midline. (D) Nkx6 is
expressed in 30 to 40 postmitotic neurons at stage 14. Note, Nkx6 expression
levels vary widely between neurons. (E-J) Wild-type embryos labeled with
indicated antibodies. (E) Nkx6 and Dpn are co-expressed in medial neuroblasts
in stage 11 embryos. Arrowhead points to a cluster of NBs including NBs 1-2,
3-1, 3-2, and 4-2. Arrow points to NB5-2. (F) Confocal projection of stage 14
CNS with Nkx6 and Eve expressed in complementary subsets of postmitotic
neurons. (G) Nkx6 and a lim3-taumyc reporter are co-expressed in the medial 1
and 3-5 RP MNs (arrowheads) at stage 14. (H-J) Confocal projection of stage 15
embryo with Nkx6 and Hb9 expressed in largely overlapping populations of
postmitotic neurons. Three segments of dissected nerve cords are shown in B-J
with anterior oriented up.
|
