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Fig. 2. Nkx6 and hb9 antagonize vnd. (A) Stage 9, (B)
stage 11, (C) stage 14 wild-type embryos stained for Nkx6 and Vnd. (A) At
stage 9 Nkx6 is expressed in midline precursor cells and Vnd is expressed in
flanking ventral neuroectoderm. (B) At stage 11 Nkx6 is expressed in clusters
of GMCs and neurons situated within stripes of Vnd-positive GMCs and neurons.
(C) At stage 14 Nkx6 and Vnd are expressed in mutually exclusive populations
of postmitotic neurons. (D) In stage 15 wild-type embryos approximately 30
neurons express Nkx6 while (E) elavGAL4:UAS-vnd embryos are devoid of
Nkx6-positive neurons. (F) In stage 15 wild-type embryos approximately ten
neurons express Vnd while (G) elavGAL4:UAS-Nkx6 mutants and (H)
elavGAL4:UAS-hb9 mutants exhibit fewer Vnd-positive neurons. (I)
Stage 15 hb9KK30 Nkx6D25 double mutant embryo
displays ectopic Vnd-positive neurons. Three segments of dissected nerve cords
with anterior up are shown in all panels.
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