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Fig. 3. Nkx6 and hb9 collaborate to regulate neuronal fate. (A) Stage 15 wild-type and (B) stage 15 elavGAL4:UAS-eve embryos stained for Nkx6. Ectopic eve expression largely abolishes Nkx6 expression. Stage 15 (C) wild-type, (D) elavGAL4:UAS-Nkx6 (E) hb9^KK30, and (F) hb9^KK30 Nkx6^D25 embryos labeled with -Eve. (D) elavGAL4:UAS-Nkx6 embryos exhibit a specific reduction of Eve expression in the U MNs and EL interneurons, but not in RP2 or a/pCC. (E) hb9 mutant displays two ectopic Eve-positive neurons per hemisegment (arrowheads). (F) hb9 Nkx6 mutant displays roughly six ectopic Eve neurons per hemisegment. (G-J) Confocal projections of stage 15 (G,I) wild-type and (H, J) hb9 Nkx6 mutant embryos stained for Lim3 (G,H) or Islet (I,J). The 1 and 3-5 RP MNs express Lim3 (G) and Islet (I) while in hb9 Nkx6 double mutants, Lim3 (H) and Islet (J) are no longer expressed in RP MNs (arrowheads). Three segments of dissected nerve cords with anterior up are shown in all panels.

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