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Fig. 5. Nkx6 overexpression drives ISNb overgrowth. (A) wild-type and (B-D) elavGAL4:2xUAS-Nkx6 embryos labeled with {alpha}-Fas2 to mark motor axons. Schematics outlining motor axon phenotypes are below each panel. Single hemisegments are shown in (A-C), in (D) adjacent hemisegments are shown. (A) In wild type, ISNb innervates ventral muscles 7, 6, 13, and 12 in a stereotyped fashion. (B-D) In Nkx6 overexpression embryos ISNb appears to exhibit exuberant growth. In (B) the terminal arbor between muscles 13 and 12 is expanded. (C) ISNb contains supernumerary branches. (D) ISNb in adjacent hemisegments have crossed the segment boundary and fused. Anterior is up in all panels.





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