|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Par-6 and Baz expression in the ovary. (A) Schematic of egg chambers at
stages 8-10. Nurse cells (nc), and oocyte (o) are indicated. Border cells
(green) are shown prior to, during and after migration. (B-M) Single confocal
sections of wild-type anterior follicle cells (B-C'), and border cell
clusters at the initiation of migration (E-F'), during migration
(H-I'), and at the completion of migration (K-L'). Schematics of
wild type stage 8 (D), early stage 9 (G), mid stage 9 (J) and stage 10 (M) egg
chambers depict the position of the border cells (red) in the adjacent
confocal images. Baz staining (green) is shown in panels (B,E,H,K) and double
labeling with rhodamine phalloidin (red) to mark F-actin in panels
(B',E',H',K'). Co-localization appears yellow. Par-6
staining (green) is shown in panels (C,F,I,L) and double labeling with
phalloidin (red) in panels (C',F',I',L'). Polar cells
(pc) are indicated in migrating clusters. High levels of Baz and Par-6
expression at border cell/border cell boundaries are indicated by arrows.
(N-P') Three-dimensional confocal reconstruction and schematic
representation of a border cell cluster in mid migration. The orientation of
the border cell cluster in panel N is identical to that of the border cell
cluster shown in panel H. Par-6 (green) and E-cadherin (red) are shown. Par-6
expression at the apices of the central polar cells (pc) is indicated by two
straight lines.
|
